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dc.creatorMercado P,Rubén
dc.creatorJercic L,María Isabel
dc.creatorTorres H,Patricio
dc.creatorAlcayaga U,Sergio
dc.creatorMartins de Paula,Fabiana
dc.creatorCosta-Cruz,Julia María
dc.creatorUeta,Marlene T
dc.date2002-12-01
dc.date.accessioned2019-04-25T13:08:23Z
dc.date.available2019-04-25T13:08:23Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98872002001200005
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/68116
dc.descriptionBackground: Strongyloides stercoralis is a world wide distributed small intestinal nematode parasite. In immunocompetent individuals S stercoralis can produce asymptomatic infections or a moderate clinical picture of diarrhea, some cases become chronic. In immunocompromised patients, a disseminated disease may appear, sometimes fatal. In Chile, there is little epidemiological information about S stercoralis infections and appropriate diagnostic techniques are usually not used. Aim: To evaluate the yield of an ELISA test for the diagnosis of strongyloidiasis in Chilean patients. Material and methods: Ten serum samples from patients with S stercoralis infections confirmed by a positive stool examination, 66 samples from individuals with other infections by tissue helminthes (24 toxocariasis, 15 trichinellosis, 11 hydatidosis, 12 fascioliasis and 4 cysticercosis), 13 samples from subjects with autoimmune diseases and 49 samples from apparently healthy individuals with a normal eosinophil count, were studied. ELISA antigen was prepared using a filariform larval extract obtained from a murine species of Strongyloides, maintained in laboratory animals. Results: Using 0.33 optical density units as a cut off value, 9 of 10 sera of S stercoralis infected individuals, had a positive ELISA test. No cross reactions were observed with sera of patients with other helminthic infections, autoimmune diseases or in healthy individuals. Thus, specificity, positive and negative predictive values were 100%. Conclusions: The results obtained are similar with those found by other investigators. ELISA test for strongyloidiasis is a useful tool for the diagnosis of clinical cases and for seroepidemiological studies of this nematode infection in Chile (Rev Méd Chile 2002; 130: 1358-64)
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dc.publisherSociedad Médica de Santiago
dc.relation10.4067/S0034-98872002001200005
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista médica de Chile v.130 n.12 2002
dc.subjectEnzyme linked immunosorbent assay
dc.subjectNematode infections
dc.subjectStrongyloides stercoralis
dc.titleInmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA


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