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DEVELOPMENT AND VALIDATION OF SIMPLE, RAPID AND SENSITIVE UV, HPLC AND HPTLC METHODS FOR THE ESTIMATION OF PIRFENIDONE IN TABLET DOSAGE FORM

dc.contributoren-US
dc.creatorThorat, S. G.
dc.creatorPadmane, S. P.
dc.creatorTajne, M. R.
dc.creatorIttadwar, A. M.
dc.date2016-06-10
dc.date.accessioned2019-09-10T17:53:35Z
dc.date.available2019-09-10T17:53:35Z
dc.identifierhttp://www.jcchems.com/index.php/JCCHEMS/article/view/26
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/108682
dc.descriptionUV, HPLC and HPTLC methods were developed and validated for the quantitative determination of Pirfenidone, a novel antifibrotic agent used in idiopathic pulmonary fibrosis. Chromatography was carried out by isocratic technique on reversed phase Eclipse XDB-C18 column (150 x 4.6 mm, 5μm) with mobile phase consisting of phosphate buff: acetonitrile (pH 3.5) 72:28 v/v at flow rate 1mL/min. TLC was carried out by stationary phase precoated Silica Gel 60 F254 TLC Plate using mobile phase Toluene: Methanol, 8:2 v/v. The UV spectrophotometric determination was performed at 311nm using solvent methanol. The proposed methods were validated according to ICH Q2-(R1) guidelines. The linearity range for Pirfenidone was 5-70 μg/mL for HPLC, 800-1600 ng/spot for HPTLC and 10-60 μg/mL for UV method. These methods were accurate and precise with recoveries in the range of 98.2-102.32 and relative standard deviation < 2%. The developed methods were successfully applied for determination of Pirfenidone in tablets.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedad Chilena de Químicaen-US
dc.relationhttp://www.jcchems.com/index.php/JCCHEMS/article/view/26/27
dc.rightsCopyright (c) 2016 Journal of the Chilean Chemical Societyen-US
dc.sourceJournal of the Chilean Chemical Society; Vol 61, No 2 (2016): Journal of the Chilean Chemical Societyen-US
dc.source0717-9707
dc.subjecten-US
dc.subjectUV; HPLC; HPTLC; Pirfenidone: Anti-fibrotic agenten-US
dc.subjecten-US
dc.titleDEVELOPMENT AND VALIDATION OF SIMPLE, RAPID AND SENSITIVE UV, HPLC AND HPTLC METHODS FOR THE ESTIMATION OF PIRFENIDONE IN TABLET DOSAGE FORMen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US


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