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DEVELOPMENT AND VALIDATION OF SIMPLE, RAPID AND SENSITIVE UV, HPLC AND HPTLC METHODS FOR THE ESTIMATION OF PIRFENIDONE IN TABLET DOSAGE FORM

dc.creatorThorat, S. G.
dc.creatorPadmane, S. P.
dc.creatorTajne, M. R.
dc.creatorIttadwar, A. M.
dc.date2016-06-10
dc.date.accessioned2019-11-13T15:11:19Z
dc.date.available2019-11-13T15:11:19Z
dc.identifierhttp://www.jcchems.com/index.php/JCCHEMS/article/view/26
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/112476
dc.descriptionUV, HPLC and HPTLC methods were developed and validated for the quantitative determination of Pirfenidone, a novel antifibrotic agent used in idiopathic pulmonary fibrosis. Chromatography was carried out by isocratic technique on reversed phase Eclipse XDB-C18 column (150 x 4.6 mm, 5μm) with mobile phase consisting of phosphate buff: acetonitrile (pH 3.5) 72:28 v/v at flow rate 1mL/min. TLC was carried out by stationary phase precoated Silica Gel 60 F254 TLC Plate using mobile phase Toluene: Methanol, 8:2 v/v. The UV spectrophotometric determination was performed at 311nm using solvent methanol. The proposed methods were validated according to ICH Q2-(R1) guidelines. The linearity range for Pirfenidone was 5-70 μg/mL for HPLC, 800-1600 ng/spot for HPTLC and 10-60 μg/mL for UV method. These methods were accurate and precise with recoveries in the range of 98.2-102.32 and relative standard deviation < 2%. The developed methods were successfully applied for determination of Pirfenidone in tablets.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedad Chilena de Químicaen-US
dc.relationhttp://www.jcchems.com/index.php/JCCHEMS/article/view/26/27
dc.rightsCopyright (c) 2016 Journal of the Chilean Chemical Societyen-US
dc.sourceJournal of the Chilean Chemical Society; Vol 61 No 2 (2016): Journal of the Chilean Chemical Societyen-US
dc.source0717-9707
dc.source0717-9324
dc.subjectUVen-US
dc.subjectHPLCen-US
dc.subjectHPTLCen-US
dc.subjectPirfenidoneen-US
dc.subjectAnti-fibrotic agenten-US
dc.titleDEVELOPMENT AND VALIDATION OF SIMPLE, RAPID AND SENSITIVE UV, HPLC AND HPTLC METHODS FOR THE ESTIMATION OF PIRFENIDONE IN TABLET DOSAGE FORMen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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