CONFIRMATION OF DEOXYNIVALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY

Vega-Herrera, Mario; Madariaga, Ricardo; Aranda, Mario; Morlock, Gertrud E.

dc.creator	Vega-Herrera, Mario
dc.creator	Madariaga, Ricardo
dc.creator	Aranda, Mario
dc.creator	Morlock, Gertrud E.
dc.date	2017-06-16
dc.date.accessioned	2019-11-13T15:11:34Z
dc.date.available	2019-11-13T15:11:34Z
dc.identifier	http://www.jcchems.com/index.php/JCCHEMS/article/view/181
dc.identifier.uri	https://revistaschilenas.uchile.cl/handle/2250/112590
dc.description	Deoxynivalenol (DON) is a mycotoxin that belongs to trichothecene subgroup B. Due its detrimental effects for human health, DON has been analyzed and detected by nearly all chromatographic methods. The present work reports for the first time the application of HPTLC/MS to confirm the presence of DON in wheat crops. Chromatography was performed on silica gel 60 F254 HPTLC plates using toluene - ethyl acetate - formic acid 6:3:1 (V/V/V) as mobile phase. After post-chromatographic derivatization with 10 % AlCl3, fluorescence detection was carried out at UV 366/>400 nm. Bands identity and purity was confirmed bymass spectrometry acquiring analytes directly from the sample bands by means of TLC/MS elution head-based interface. Both, sample and standard bands showed clear mass signals at m/z 341 that corresponds to the formate adduct of DON [M+HCOO]-. Thus, the presence of DON in the Chilean wheat sample was confirmed.	en-US
dc.format	application/pdf
dc.language	eng
dc.publisher	Sociedad Chilena de Química	en-US
dc.relation	http://www.jcchems.com/index.php/JCCHEMS/article/view/181/143
dc.rights	Copyright (c) 2017 Mario Vega-Herrera, Ricardo Madariaga, Mario Aranda, Gertrud E. Morlock	en-US
dc.rights	https://creativecommons.org/licenses/by-nc-sa/4.0	en-US
dc.source	Journal of the Chilean Chemical Society; Vol 62 No 2 (2017): Journal of the Chilean Chemical Society	en-US
dc.source	0717-9707
dc.source	0717-9324
dc.subject	Deoxynivalenol	en-US
dc.subject	mycotoxins	en-US
dc.subject	HPTLC/MS	en-US
dc.subject	Fusarium	en-US
dc.subject	TLC	en-US
dc.title	CONFIRMATION OF DEOXYNIVALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY	en-US
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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