| dc.creator | Da Silva, Paulo A. B. | |
| dc.creator | De Souza, Gustavo C. S. | |
| dc.creator | Paim, Ana Paula S. | |
| dc.creator | Lavorante, André F. | |
| dc.date | 2018-06-25 | |
| dc.date.accessioned | 2019-11-13T15:11:47Z | |
| dc.date.available | 2019-11-13T15:11:47Z | |
| dc.identifier | http://www.jcchems.com/index.php/JCCHEMS/article/view/684 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/112688 | |
| dc.description | A multicommuted flow procedure for the spectrophotometric determination of reducing sugars in wine has been developed. A dialysis unit was incorporated in the system to minimize matrix interferences and eliminate the colored compounds of the wine. The oxidation-reduction reaction in an alkaline medium between reducing sugars and the Cu2+-Neocuproine complex was employed. The compound formed was monitored at 460 nm. In the proposed procedure, there was a linear response between 0.25 and 4.0 g L-1 reducing sugars (y = (0.36 ± 0.02) + (0.31 ± 0.01) * x, R = 0.9975, n = 6), a coefficient of variance of 2.6% for a reducing sugar solution of 2.0 g L-1 (n = 7), a limit of detection estimated at 0.03 g L-1, reagent consumption per determination of 0.330, 0.128 and 0.160 mg for neocuproine, CuSO4 and NaOH, respectively. A sampling throughput of 45 determinations per hour was achieved. The proposed system was applied to wine samples and comparing the results with an AOAC official method no significant difference at a 95% confidence level. | en-US |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Sociedad Chilena de Química | en-US |
| dc.relation | http://www.jcchems.com/index.php/JCCHEMS/article/view/684/242 | |
| dc.rights | Copyright (c) 2018 Journal of the Chilean Chemical Society | en-US |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/4.0 | en-US |
| dc.source | Journal of the Chilean Chemical Society; Vol 63 No 2 (2018): Journal of the Chilean Chemical Society | en-US |
| dc.source | 0717-9707 | |
| dc.source | 0717-9324 | |
| dc.subject | Dialysis | en-US |
| dc.subject | Flow injection analysis | en-US |
| dc.subject | Multicommutation | en-US |
| dc.subject | Reducing sugar | en-US |
| dc.subject | Wine | en-US |
| dc.title | SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH | en-US |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
