ONE-STEP PURIFICATION OF TWO SEMI-SYNTHETIC EPICATECHIN ADDUCTS PREPARED FROM AVOCADO PEELS PROCYANIDINS BY CENTRIFUGAL PARTITION CHROMATOGRAPHY AND EVALUATION OF THEIR ANTI-INFLAMMATORY EFFECTS ON ADENOCARCINOMA GASTRIC CELLS INFECTED WITH Helicobacter pylori

Torres, Eillen; García, Apolinaria; Aranda, Mario; Saéz, Vania; Zúñiga, Felipe; Alarcón, Julio; Avello, Marcia; Pastene, Edgar

dc.creator	Torres, Eillen
dc.creator	García, Apolinaria
dc.creator	Aranda, Mario
dc.creator	Saéz, Vania
dc.creator	Zúñiga, Felipe
dc.creator	Alarcón, Julio
dc.creator	Avello, Marcia
dc.creator	Pastene, Edgar
dc.date	2019-01-09
dc.date.accessioned	2019-11-13T15:11:50Z
dc.date.available	2019-11-13T15:11:50Z
dc.identifier	http://www.jcchems.com/index.php/JCCHEMS/article/view/920
dc.identifier.uri	https://revistaschilenas.uchile.cl/handle/2250/112726
dc.description	Avocado peels were used to extract proanthocyanidins (PACs) as starting material to prepare semi-synthetic derivatives of epicatechin by nucleophilic attack with pyrogallol and resorcinol. One-step isolation of both semi-synthetic derivatives was successfully performed through centrifugal partition chromatography (CPC), and identified by TLC and LC-MS/MS. The effect upon adherence of Helicobacter pylori ATCC 43504 to AGS cells and the subsequent induction of pro-inflammatory IL-8 release was tested for these two semi-synthetic derivatives. Bacterial adherence and IL-8 synthesis was evaluated using AGS cells con-infected with FITC-labeled H. pylori. Concentration of 700 μg/mL of epicatechin-pyrogallol and epicatechin-resorcinol decreased H. pylori adherence to AGS cells to less than 20% and reduced IL-8 production to less than 10%. In conclusion, semi-synthetic adducts prepared from avocado PACs reduce significantly the adherence and IL-8 production in H. pylori-infected AGS cells.	en-US
dc.format	application/pdf
dc.language	eng
dc.publisher	Sociedad Chilena de Química	en-US
dc.relation	http://www.jcchems.com/index.php/JCCHEMS/article/view/920/281
dc.rights	Copyright (c) 2019 Journal of the Chilean Chemical Society	en-US
dc.rights	https://creativecommons.org/licenses/by-nc-sa/4.0	en-US
dc.source	Journal of the Chilean Chemical Society; Vol 63 No 4 (2018): Journal of the Chilean Chemical Society	en-US
dc.source	0717-9707
dc.source	0717-9324
dc.subject	Centrifugal Partition Chromatography	en-US
dc.subject	Helicobacter pylori	en-US
dc.subject	avocado	en-US
dc.subject	proanthocyanidins	en-US
dc.subject	IL-8	en-US
dc.title	ONE-STEP PURIFICATION OF TWO SEMI-SYNTHETIC EPICATECHIN ADDUCTS PREPARED FROM AVOCADO PEELS PROCYANIDINS BY CENTRIFUGAL PARTITION CHROMATOGRAPHY AND EVALUATION OF THEIR ANTI-INFLAMMATORY EFFECTS ON ADENOCARCINOMA GASTRIC CELLS INFECTED WITH Helicobacter pylori	en-US
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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