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dc.creatorTorres, Eillen
dc.creatorGarcía, Apolinaria
dc.creatorAranda, Mario
dc.creatorSaéz, Vania
dc.creatorZúñiga, Felipe
dc.creatorAlarcón, Julio
dc.creatorAvello, Marcia
dc.creatorPastene, Edgar
dc.date2019-01-09
dc.date.accessioned2019-11-13T15:11:50Z
dc.date.available2019-11-13T15:11:50Z
dc.identifierhttp://www.jcchems.com/index.php/JCCHEMS/article/view/920
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/112726
dc.descriptionAvocado peels were used to extract proanthocyanidins (PACs) as starting material to prepare semi-synthetic derivatives of epicatechin by nucleophilic attack with pyrogallol and resorcinol. One-step isolation of both semi-synthetic derivatives was successfully performed through centrifugal partition chromatography (CPC), and identified by TLC and LC-MS/MS. The effect upon adherence of Helicobacter pylori ATCC 43504 to AGS cells and the subsequent induction of pro-inflammatory IL-8 release was tested for these two semi-synthetic derivatives. Bacterial adherence and IL-8 synthesis was evaluated using AGS cells con-infected with FITC-labeled H. pylori. Concentration of 700 μg/mL of epicatechin-pyrogallol and epicatechin-resorcinol decreased H. pylori adherence to AGS cells to less than 20% and reduced IL-8 production to less than 10%. In conclusion, semi-synthetic adducts prepared from avocado PACs reduce significantly the adherence and IL-8 production in H. pylori-infected AGS cells.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherSociedad Chilena de Químicaen-US
dc.relationhttp://www.jcchems.com/index.php/JCCHEMS/article/view/920/281
dc.rightsCopyright (c) 2019 Journal of the Chilean Chemical Societyen-US
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0en-US
dc.sourceJournal of the Chilean Chemical Society; Vol 63 No 4 (2018): Journal of the Chilean Chemical Societyen-US
dc.source0717-9707
dc.source0717-9324
dc.subjectCentrifugal Partition Chromatographyen-US
dc.subjectHelicobacter pylorien-US
dc.subjectavocadoen-US
dc.subjectproanthocyanidinsen-US
dc.subjectIL-8en-US
dc.titleONE-STEP PURIFICATION OF TWO SEMI-SYNTHETIC EPICATECHIN ADDUCTS PREPARED FROM AVOCADO PEELS PROCYANIDINS BY CENTRIFUGAL PARTITION CHROMATOGRAPHY AND EVALUATION OF THEIR ANTI-INFLAMMATORY EFFECTS ON ADENOCARCINOMA GASTRIC CELLS INFECTED WITH Helicobacter pylorien-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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