| dc.creator | Ali,Sikander | |
| dc.creator | ul-Haq,Ikram | |
| dc.creator | Qadeer,M.A. | |
| dc.date | 2002-08-01 | |
| dc.date.accessioned | 2019-11-14T12:56:05Z | |
| dc.date.available | 2019-11-14T12:56:05Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200006 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/117512 | |
| dc.description | L-DOPA is a useful drug for Parkinson s disease. This investigation deals with the biosynthesis of L-DOPA by parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae. There was a marked difference between the mycelial morphology and pellet type of the parental and UV-irradiated mutant cultures. The mutant strain of Aspergillus oryzae UV-6 showed pellet-like mycelial morphology and improved tyrosinase activity. Mould mycelium was used for biochemical conversion of L-tyrosine to L-DOPA since tyrosinase is an intracellular enzyme. The mutant was found to give 3.72 folds higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed greater ability of the mutant to yield L-DOPA (i.e. Yp/x 32.73 mg/mg with parent and 95.71 mg/mg with mutant). When cultures grown for various incubation periods, were monitored for Qp, Qs and q p, there was significant enhancement (p<0.0025-0.005) in these variables by the mutant strain of Aspergillus oryzae UV-7 over GCB-6 on all the rates. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | 10.4067/S0717-34582002000200006 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.5 n.2 2002 | |
| dc.title | Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae | |
