| dc.creator | Naito,Yuji | |
| dc.creator | Shimozawa,Makoto | |
| dc.creator | Manabe,Hiroki | |
| dc.creator | Kuroda,Masaaki | |
| dc.creator | Tomatsuri,Naoya | |
| dc.creator | Uchiyama,Kazuhiko | |
| dc.creator | Takagi,Tomohisa | |
| dc.creator | Yoshida,Norimasa | |
| dc.creator | Yoshikawa,Toshikazu | |
| dc.date | 2004-01-01 | |
| dc.date.accessioned | 2019-11-14T12:59:41Z | |
| dc.date.available | 2019-11-14T12:59:41Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200009 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/119610 | |
| dc.description | Red wine polyphenolic compounds have been demonstrated to possess antioxidant properties, and several studies have suggested that they might constitute a relevant dietary factor in the protection from coronary heart disease. The aim of the present study is to examine whether red wine extracts (RWE) can ameliorate oxysterol-induced endothelial response, and whether inhibition of adhesion molecule expression is involved in monocyte adhesion to endothelial cells. Surface expression and mRNA levels of adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1) were determined by ELISA and RT-PCR performed on human aortic endothelial cells (HAEC) monolayers stimulated with 7b-hydroxycholesterol or 25-hydroxycholesterol. Incubation of HAEC with oxysterols (10 muM) increased expression of adhesion molecules in a time-dependent manner. Pretreatment of HAEC with RWE at final concentrations of 1, 10, and 100 ng/ml significantly inhibited the increase of surface protein expression and mRNA levels. Adherence of monocytes to oxysterol-stimulated HAEC was increased compared to that of unstimulated cells. Treatment of HAEC with RWE significantly inhibited adherence of monocytes. These results suggest that RWE works as an anti-atherogenic agent through the inhibition of endothelial-dependent adhesive interactions with monocytes induced by oxysterols | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602004000200009 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.37 n.2 2004 | |
| dc.subject | Adhesion molecule | |
| dc.subject | monocyte-endothelial cell interaction | |
| dc.subject | oxysterol | |
| dc.subject | red wine extract | |
| dc.title | Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols | |
