| dc.creator | Pavón-Pérez, Jessy | |
| dc.creator | Oviedo, Claudia | |
| dc.creator | Elso-Freudenberg, Mhartyn | |
| dc.creator | Henriquez-Aedo, Karem | |
| dc.creator | Aranda, Mario | |
| dc.date | 2019-12-18 | |
| dc.date.accessioned | 2020-01-06T20:13:36Z | |
| dc.date.available | 2020-01-06T20:13:36Z | |
| dc.identifier | http://www.jcchems.com/index.php/JCCHEMS/article/view/1332 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/122369 | |
| dc.description | A rapid, sensitive, precise and accurate liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for levo 3,4-dihydroxyphenylalanine (L-DOPA) determination in Vicia faba during different growth stages. The method applied a simple sample preparation step followed by a chromatographic separation on a Kinetex XB Core-Shell C18 (100 mm x 4.6 mm, 2.6 µm) column, using a mixture of ultrapure pure water (A) with 0.5 % (v/v) formic acid and methanol (B) as mobile phase. Analysis of L-DOPA was carried out by MS/MS applying a Multiple Reaction Monitoring (MRM) method using the transition m/z 198 → m/z 152. This LC-MS/MS method allowed a well-resolved detection of L-DOPA in ca. 2 min within 6 min run. Method was validated showing a linear range from 0.05 to 10 mg L-1 (R2 = 0.99); repeatability showed RSD value of 1.40%. Recoveries ranged from 94.14 to 116.62% with RSD values ≤ 5.66% and detection and quantification limits were 0.01 and 0.05 mg L-1, respectively. Applying this validated method, L-DOPA was determined in Vicia faba samples to determined its tissue distribution. As expected, a broad range of L-DOPA content finding values from 4.72 (in seeds) up to 133.60 mg g-1 (sprouts).
| en-US |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Sociedad Chilena de Química | en-US |
| dc.relation | http://www.jcchems.com/index.php/JCCHEMS/article/view/1332/356 | |
| dc.rights | Copyright (c) 2019 Journal of the Chilean Chemical Society | en-US |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/4.0 | en-US |
| dc.source | Journal of the Chilean Chemical Society; Vol 64 No 4 (2019): Journal of the Chilean Chemical Society; 4651-4653 | en-US |
| dc.source | 0717-9707 | |
| dc.source | 0717-9324 | |
| dc.subject | mass spectrometry | en-US |
| dc.subject | L-DOPA | en-US |
| dc.subject | Vicia faba | en-US |
| dc.subject | LC-MS/MS | en-US |
| dc.title | LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF Vicia faba | en-US |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
