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dc.creatorSepúlveda A,Marcela
dc.creatorBello T,Helia
dc.creatorRuiz C,Mery
dc.creatorHormazábal F,José
dc.creatorDomínguez Y,Mariana
dc.creatorGonzález R,Gerardo
dc.creatorMella M,Sergio
dc.creatorZemelman Z,Raúl
dc.date2002-01-01
dc.date.accessioned2020-02-17T15:27:15Z
dc.date.available2020-02-17T15:27:15Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000100006
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/127243
dc.descriptionBackground: Enterococcus is a bacterial genus with low virulence. However, in the last years, the importance of some enterococcus species as nosocomial pathogens has increased, specially due to their resistance to some antimicrobial. Aim: To identify enterococcus strains using classical biochemical techniques and genomic amplification with Polymerase Chain Reaction (PCR). Material and methods: Three hundred and five enterococcus strains, isolated between 1996 and 1999, from different clinical specimens in hospitals and other centers of the VIIIth Region of Chile, were studied. The isolates were identified, to the species level, according to the scheme proposed by Carvalho et al. Identification of some strains was confirmed by PCR. Results: Eighty nine percent of isolates were identified as E fæcalis, 10.2% as E fæcium and 3.3% as other species. Conclusions: PCR is a fast and promising technique, useful in the identification of Enterococcus species (Rev Méd Chile 2002; 130: 45-49)
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dc.publisherSociedad Médica de Santiago
dc.relation10.4067/S0034-98872002000100006
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista médica de Chile v.130 n.1 2002
dc.subjectEnterococcus
dc.subjectEnterococcus fæcalis
dc.subjectEnterococcus fæcium
dc.subjectPolymerase chain reaction
dc.titleMetodología clásica y molecular en la identificación de especies de Enterococcus spp


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