| dc.creator | FAROUDI,MUSTAPHA | |
| dc.creator | ZARU,ROSSANA | |
| dc.creator | FAVIER,BENOIT | |
| dc.creator | VALITUTTI,SALVATORE | |
| dc.date | 2002-01-01 | |
| dc.date.accessioned | 2020-02-17T15:28:41Z | |
| dc.date.available | 2020-02-17T15:28:41Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200004 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/128079 | |
| dc.description | Three innovative and complementary morphological approaches were employed to study the T cell/antigen presenting cell (APC) interaction: (i) high resolution three-dimensional confocal microscopy of the T cell-APC contact site; (ii) time lapse video recording in living T cells of [Ca2+]I and changes in distribution of various GFP fusion proteins with TCR/CD3-zetacomplex associated- and other signaling components; (iii) measurement of lateral TCR mobility and that of recruited signaling components using techniques based on fluorescence recovery after photo-bleaching. These approaches were combined with biochemical and functional experiments to investigate two principal issues: (A) Recruitment and the three-dimensional arrangement of receptors and signaling components at the contact site between human CD4+ T lymphocytes and APCs, (B) Structure of the immunological synapse formed at the contact site between cytotoxic T lymphocytes (CTLs) and target cells. We discuss evidence indicating that TCR engagement and triggering can occur in the absence of large-scale molecular segregation into the T cell-APC contact site. Taken together our results indicate that although not required for TCR engagement and triggering, formation of the IS is important to reinforce TCR-mediated signal transduction and achieve full T cell activation. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602002000200004 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.35 n.2 2002 | |
| dc.subject | T cell antigen receptor | |
| dc.subject | Immunological synapse | |
| dc.subject | T cell antigen receptor | |
| dc.subject | signal transduction | |
| dc.title | New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse | |
