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dc.creatorJAIMOVICH,ENRIQUE
dc.creatorCARRASCO,MARIA ANGELICA
dc.date2002-01-01
dc.date.accessioned2020-02-17T15:28:42Z
dc.date.available2020-02-17T15:28:42Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/128085
dc.descriptionPotassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including results obtained using inhibitors specific for individual steps, allowed us to connect these in a sequential manner. As the same type of signaling cascade can be triggered by oxidants, neurotransmitters and hormones, the ensemble of results allows us to propose a general model to describe signaling events that link membrane stimulation to regulation of gene transcription in skeletal muscle cells
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dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602002000200010
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.35 n.2 2002
dc.subjectExcitation-transcription
dc.subjectinositol trisphosphate
dc.subjectmitogen-activated kinases
dc.subjectCREB
dc.subjectearly genes
dc.subjectdihydropyridine receptors
dc.titleIP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression


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