dc.creator | JAIMOVICH,ENRIQUE | |
dc.creator | CARRASCO,MARIA ANGELICA | |
dc.date | 2002-01-01 | |
dc.date.accessioned | 2020-02-17T15:28:42Z | |
dc.date.available | 2020-02-17T15:28:42Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010 | |
dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/128085 | |
dc.description | Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including results obtained using inhibitors specific for individual steps, allowed us to connect these in a sequential manner. As the same type of signaling cascade can be triggered by oxidants, neurotransmitters and hormones, the ensemble of results allows us to propose a general model to describe signaling events that link membrane stimulation to regulation of gene transcription in skeletal muscle cells | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Sociedad de Biología de Chile | |
dc.relation | 10.4067/S0716-97602002000200010 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Biological Research v.35 n.2 2002 | |
dc.subject | Excitation-transcription | |
dc.subject | inositol trisphosphate | |
dc.subject | mitogen-activated kinases | |
dc.subject | CREB | |
dc.subject | early genes | |
dc.subject | dihydropyridine receptors | |
dc.title | IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression | |