| dc.creator | SIMON,FELIPE | |
| dc.creator | VARELA,DIEGO | |
| dc.creator | RIVEROS,ANA | |
| dc.creator | EGUIGUREN,ANA LUISA | |
| dc.creator | STUTZIN,ANDRES | |
| dc.date | 2002-01-01 | |
| dc.date.accessioned | 2020-02-17T15:28:42Z | |
| dc.date.available | 2020-02-17T15:28:42Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200013 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/128088 | |
| dc.description | Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transporters. A specific mechanism of swelling and necrosis driven by the influx of Na+ through nonselective cation channels has been recently proposed (<A HREF="#2">Barros et al., 2001a</A>). We have characterized further the properties of the nonselective cation channel (NSCC) in HTC cells. The NSCC shows a conductance of ~18 pS, is equally permeable to Na+ and K+, impermeant to Ca2+, requires high intracellular Ca2+ as well as low intracellular ATP for activation and is inhibited by flufenamic acid. Hydrogen peroxide induced a significant increase in cell volume that was dependent on external Na+. We propose that the NSCC, which is ubiquitous though largely inactive in healthy cells, becomes activated under severe oxidative stress. The ensuing Na+ influx initiates via positive feedback a series of metabolic and electrolytic disturbances, resulting in cell death by necrosis | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602002000200013 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.35 n.2 2002 | |
| dc.subject | Cell death | |
| dc.subject | Cell Volume | |
| dc.subject | Nonselective cation channels | |
| dc.subject | Flufenamic acid | |
| dc.title | Non-selective cation channels and oxidative stress- induced cell swelling | |
