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dc.creatorPanichayupakaranant,Pharkphoom
dc.creatorTewtrakul,Supinya
dc.date2002-12-01
dc.date.accessioned2020-02-17T15:30:15Z
dc.date.available2020-02-17T15:30:15Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300006
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/128973
dc.descriptionRoot cultures of Plumbago rosea Linn. were established from young leaf explants on solid Gamborg's B5 (B5) medium supplemented with the combination of <FONT FACE=Symbol>á</FONT>-naphthalene acetic acid (NAA) and kinetin in the concentration ranges of 0.5-2.0 mg/l and 0.1-0.5 mg/l, respectively. The production of plumbagin, determined by TLC-densitometry was higher [0.016 ± 0.0030% dry weight (DW)] in cultured roots obtained from B5 medium supplemented with 1.0 mg/l NAA and 0.1 mg/l kinetin. Plant selection increased the plumbagin production to 0.129 ± 0.0139% DW, while variation of sucrose and nitrogen (as (NH4)2SO4) concentration in B5 media slightly increase the plumbagin synthesis to 0.023 ± 0.0017 and 0.020 ± 0.0015% DW, respectively.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.5 n.3 2002
dc.titlePlumbagin production by root cultures of Plumbago rosea


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