| dc.creator | Bollati Fogolín,Mariela | |
| dc.creator | Eberhardt,Marcos Oggero | |
| dc.creator | Kratje,Ricardo | |
| dc.creator | Etcheverrigaray,Marina | |
| dc.date | 2002-12-01 | |
| dc.date.accessioned | 2020-02-17T15:30:15Z | |
| dc.date.available | 2020-02-17T15:30:15Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300008 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/128975 | |
| dc.description | Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein content, and therefore useful for determining concentrations of the cytokine in production processes. Among these assays, the competitive ELISA, developed with a MAb that recognises an epitope not involved in glycosylation, was the only one suitable to quantify the glycosylated form of rhGM-CSF produced in mammalian cell cultures. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.5 n.3 2002 | |
| dc.title | Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems | |
