| dc.creator | VISSE,EDWARD | |
| dc.creator | INOSTROZA,JUAN | |
| dc.creator | CABELLO,GERTRUDIS | |
| dc.creator | PARRA,EDUARDO | |
| dc.date | 2003-01-01 | |
| dc.date.accessioned | 2020-02-17T15:30:54Z | |
| dc.date.available | 2020-02-17T15:30:54Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200016 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/129353 | |
| dc.description | To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/B7-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-g and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and CD2/LFA-3 costimulation resulted in the up-regulation of IL-4 and IFN-g promoters, IL-2 promoter activity and production of IL-2 were only seen after B7-1 costimulation. However, contrary to what has been previously proposed, we show that costimulation with either B7-1 or LFA-3 further enhanced the ERK-2 activity and strongly activated the p38 MAPK pathway, but only B7-1 costimulation induced high levels of JNK-1 activity. These data suggest that the differential effect of CD28 vs. CD2 can be related to the difference in the ability of the two pathways to induce JNK-1 activity. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602003000200016 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.36 n.2 2003 | |
| dc.subject | CD28 response element | |
| dc.subject | Staphylococcal Enterotoxin A-E | |
| dc.subject | Extracellular signal regulated kinase | |
| dc.subject | c-Jun N-terminal kinase | |
| dc.subject | Interleukin-2 | |
| dc.title | The MAP Kinases are Differently Utilized by CD28 and CD2 Adhesion Pathways in Superantigen-Activated Jurkat T cells | |
