| dc.creator | GÖTTE,MARTIN | |
| dc.creator | STADTBÄUMER,ANDREA | |
| dc.date | 2002-01-01 | |
| dc.date.accessioned | 2020-02-17T15:30:56Z | |
| dc.date.available | 2020-02-17T15:30:56Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300009 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/129363 | |
| dc.description | The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cerevisiae to elucidate the role of the syntaxin 6-dependent vesicular trafficking step in yeast. Immunofluorescence microscopy revealed a punctate, Golgi-like staining pattern for syntaxin 6, which only partially overlapped with Pep12p in wild-type yeast cells. In contrast to Pep12p, syntaxin 6 was not mislocalized to the vacuole upon expression from 2 micron vectors, which might be attributed to conserved sorting and retention signals. Syntaxin 6 was not capable of complementing the sorting and maturation defects of the vacuolar hydrolase CPY in pep12 null mutants. No dominant negative effects of either syntaxin 6 or syntaxin 6deltaC overexpression on CPY sorting and maturation were observed in wild-type yeast cells. We conclude that syntaxin 6 and Pep12p do not act at the same vesicular trafficking step(s) in yeast and higher eukaryotes | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602002000300009 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.35 n.3-4 2002 | |
| dc.subject | vesicular transport | |
| dc.subject | SNARE | |
| dc.subject | syntaxin | |
| dc.subject | endosomal trafficking | |
| dc.subject | vacuole protein sorting | |
| dc.subject | PEP12 | |
| dc.title | Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae | |
