| dc.creator | NAIR,MADHAVAN P | |
| dc.creator | KANDASWAMI,CHITHAN | |
| dc.creator | MAHAJAN,SUPRIYA | |
| dc.creator | NAIR,HARIKRISHNA N | |
| dc.creator | CHAWDA,RAM | |
| dc.creator | SHANAHAN,THOMAS | |
| dc.creator | SCHWARTZ,STANLEY A | |
| dc.date | 2002-01-01 | |
| dc.date.accessioned | 2020-02-17T15:30:57Z | |
| dc.date.available | 2020-02-17T15:30:57Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300016 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/129370 | |
| dc.description | Flavonoids and related polyphenols, in addition to their cardioprotective, anti-tumor, anti-inflammatory, anti-carcinogenic and anti-allergic activities, also possess promising anti-HIV effects. Recent studies documented that the ß-chemokine receptors, CCR2b, CCR3 and CCR5, and the alpha-chemokine receptors, CXCR1, CXCR2 and CXCR4 serve as entry coreceptors for HIV-1. Although flavonoids and polyphenolic compounds elicit anti-HIV effects such as inhibition of HIV-1 expression and virus replication, the molecular mechanisms underlying these effects remain to be clearly elucidated. We hypothesize that flavonoids exert their anti-HIV effects, possibly by interfering at the HIV co-receptor level. We investigated the effect of flavonoid constituents of a proprietary grape seed extract (GSE) on the expression of HIV-1 coentry receptors by immunocompetent mononuclear leukocytes. Our results showed that GSE significantly downregulated the expression of the HIV-1 entry co-receptors, CCR2b , CCR3 and CCR5 in normal PBMC in a dose dependent manner. Further , GSE treated cultures showed significantly lower number of CCR3 positive cells as quantitated by flow cytometry analysis which supports RT-PCR gene expression data.Investigations of the mechanisms underlying the anti-HIV-1 effects of grape seed extracts may help to identify promising natural products useful in the prevention and /or amelioration of HIV-1 infection | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602002000300016 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.35 n.3-4 2002 | |
| dc.subject | PBMC, peripheral blood mononuclear cells | |
| dc.subject | RT- PCR - reverse transcriptase polymerase chain reaction | |
| dc.subject | HIV-1 , human immunodeficiency virus-1 GSE,- Grape Seed Extract | |
| dc.title | Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells | |
