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dc.creatorAlvarez,Maricel
dc.creatorHärtel,Steffen
dc.creatorGodoy,Roberto
dc.creatorHeyser,Wolfgang
dc.date2003-01-01
dc.date.accessioned2020-02-17T15:31:24Z
dc.date.available2020-02-17T15:31:24Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-66432003000100007
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/129625
dc.descriptionWe present a method to localise and quantify surface-bound phosphatase activity (SBPA) in mycorrhizal fungi and mycorrhizae of Nothofagus obliqua (Mirb.) Oerst., using image processed confocal fluorescent microscopy. ELF-97 is a hydrophilic substrate which turns into a strongly fluorescent precipitate upon activation by phosphomonoesterases. In fungal mycelium, this technique has recently been approved by comparison with a standard method (p-nitrophenyl-phosphate). The microscopic technique based on ELF-97 revealed that Paxillus involutus (Batsch: Fr.) Sing. and Austropaxillus boletinoides (Sing.) Brsky. & Jarosch provide different adaptive strategies to changing phosphate concentrations and different pH (3-7). We also analysed SBPA in four mycorrhizal associations of N. obliqua and found that the organisation of the mantle played an essential role regarding the SBPA. In general, mycorrhizal roots shifted SBPA from the root to the mantle. In this context, Pisolithus tinctorius (Pers.) Coker & Couch proved to be the most relevant mycorrhiza partner for N. obliqua by increasing significantly the overall SBPA of the mycorrhiza in respect to non-mycorrhizal roots. In conclusion, ELF-97 fluorescence microscopy in combination with image processing routines determined SBPA and revealed phosphorus (P) adaptation strategies of mycorrhizal fungi and mycorrhizae on a structural-physiological level
dc.formattext/html
dc.languageen
dc.publisherFacultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
dc.relation10.4067/S0717-66432003000100007
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceGayana. Botánica v.60 n.1 2003
dc.subjectConfocal microscopy
dc.subjectectomycorrhizae of Nothofagus obliqua
dc.subjectELF-97
dc.subjectphosphatase activity
dc.subjectquantitative fluorescence microscopy
dc.titleNEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE


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