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dc.creatorWILHELM,VIVIAN
dc.creatorHUARACÁN,BERNARDO
dc.creatorMARTÍNEZ,RODRIGO
dc.creatorROSEMBLATT,MARIO
dc.creatorBURZIO,LUIS O
dc.creatorVALENZUELA,PABLO D.T
dc.date2003-01-01
dc.date.accessioned2020-02-17T15:32:42Z
dc.date.available2020-02-17T15:32:42Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300013
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/130350
dc.descriptionThe genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair fragment encoding a protein of 156 aminoacids. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from other prokaryotic organisms. Both proteins were expressed in E. coli as fusion proteins with thioredoxin and purified by chromatography on Ni-column. A rabbit serum against P. salmonis total proteins reacts with the recombinant HSP10 and HSP16 proteins. Similar reactivity was determined by ELISA using serum from salmon infected with P. salmonis. The possibility of formulating a vaccine containing these two proteins is discussed
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dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602003000300013
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.36 n.3-4 2003
dc.titleCloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis


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