| dc.creator | Araya O,Juan Carlos | |
| dc.creator | Roa S,Juan Carlos | |
| dc.creator | Villaseca H,Miguel Angel | |
| dc.creator | Roa E,Iván | |
| dc.creator | Guzmán G,Pablo | |
| dc.creator | Alvarado C,César | |
| dc.date | 2003-04-01 | |
| dc.date.accessioned | 2020-02-17T15:33:28Z | |
| dc.date.available | 2020-02-17T15:33:28Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000400002 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/130785 | |
| dc.description | Background: In the current model for the development of gastric cancer, regions of multifocal atrophic gastritis give rise to intestinal metaplasia, dysplasia and finally, adenocarcinoma. Aim: To study the frequency and characteristics of TP53 gene mutations in preneoplastic and neoplastic lesions of the stomach. Material and methods: DNA sequencing of the TP53 gene was performed in 46 patients with gastric carcinoma. Normal mucosa, intestinal metaplasia and invasive adenocarcinoma tissues were obtained by scraping 6-µm histological sections from formalin-fixed and paraffin-embedded tissue. Results: DNA sequencing of exons 5-9 of the TP53 gene demonstrated a mutation in 31% of patients. These findings were seen both in tumoral tissue (13 cases) and in intestinal metaplasia (2 cases). Most mutations were found in exons 5 and 8, and the majority of them were transitions (10 out of 19 mutations). Discussion: Patients with gastric cancer showed a frequency of TP53 mutations similar to that previously communicated in populations with low gastric cancer risk. Moreover, there was a predominance of transitions, genetic alterations that are identified with carcinogenesis associated with N-nitrosamine compounds. Finally, mutations of TP53 gene were detected in areas of intestinal metaplasia (Rev Méd Chile 2003; 131: 359-65). | |
| dc.format | text/html | |
| dc.language | es | |
| dc.publisher | Sociedad Médica de Santiago | |
| dc.relation | 10.4067/S0034-98872003000400002 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Revista médica de Chile v.131 n.4 2003 | |
| dc.subject | Adenocarcinoma | |
| dc.subject | Genes | |
| dc.subject | supressor | |
| dc.subject | tumor | |
| dc.subject | Metaplasia | |
| dc.title | Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region | |
