| dc.creator | Muñoz A,Jeannette | |
| dc.creator | Bello T,Helia | |
| dc.creator | Domínguez Y,Mariana | |
| dc.creator | Mella M,Sergio | |
| dc.creator | Zemelman Z,Raúl | |
| dc.creator | González R,Gerardo | |
| dc.date | 2003-07-01 | |
| dc.date.accessioned | 2020-02-17T15:33:34Z | |
| dc.date.available | 2020-02-17T15:33:34Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000700003 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/130838 | |
| dc.description | Background: The resistance of Shigella flexneri to antimicrobial agents can be associated to the presence of integrons that may contain and express antimicrobial resistance gene cassettes. Aim: To study antimicrobial resistance and the presence of integrons and antimicrobial gene cassettes in Shigella flexneri strains. Material and methods: In vitro susceptibility to 27 antimicrobials was studied in twenty four Shigella flexneri strains isolated from stools. The presence of integrons class 1, 2 and 3 and antimicrobial resistance gene cassettes was investigated by polymerase chain reaction (PCR) using specific primers for each gene. Results: Most strains were resistant to one of the following antimicrobials: ampicillin, sulphonamide, trimethoprim, tetracycline, streptomycin, sulfamethoxazole-trimethoprim or chloramphenicol. Twenty nine percent were simultaneously resistant to all these antimicrobials. Integrons class 1 and 2 were found in 19 strains (79%). Class 3 integrons were not found. Gene cassettes dfrA1 and ant(3")I were associated to integrons class 2 in most strains (15/20, 75%). Genes cat, tetB and blarTEM were detected in 18/24 (75%), 7/24 (29%) and 4/24 (17%) of the strains, respectively and were not associated to any of the studied integrons. Genes that codify enzymes AAC(6')Ib and APH(3')VI were not detected in any strain. Conclusions: The high frequency of integrons found in the studied strains, could partly explain the increasing antimicrobial resistance of Shigella flexneri strains, isolated in Chile (Rev Méd Chile 2003; 131: 727-33) | |
| dc.format | text/html | |
| dc.language | es | |
| dc.publisher | Sociedad Médica de Santiago | |
| dc.relation | 10.4067/S0034-98872003000700003 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Revista médica de Chile v.131 n.7 2003 | |
| dc.subject | Drug resistance, microbial | |
| dc.subject | Gene library | |
| dc.subject | Integrons | |
| dc.subject | Shigella flexneri | |
| dc.title | Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri | |
