| dc.creator | Espinoza-Navarro,Omar | |
| dc.creator | Bustos-Obregón,Eduardo | |
| dc.date | 2004-12-01 | |
| dc.date.accessioned | 2020-02-17T15:34:21Z | |
| dc.date.available | 2020-02-17T15:34:21Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400010 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/131278 | |
| dc.description | Malathion is an organophosphorate insecticide whose main acute poisonous effect is the inhibition of the enzyme acetylcholinesterase. This pesticide is widely used and has been reported to damage mice spermatogenesis. The objective of this work was to evaluate the toxic risk of malathion, earthworm Eisenia foetida (Annelida, Oligochaeta) like biological model was used and testicles and spermatozoa of animal controls and treaties were analyzed. Earthworms with developed clitellum were used and they were dewed with sublethal single doses of 0, 80, 150, 300 and 600mg of malathion for kilogram of soil (LD50 = 880mg/kg dry soil). Control groups were added only distilled water. Each group was analyzed 1, 5, 15 and 30 days post treatment. The results observed in treated animals indicate a significant rise of the number of spermatozoa at 1 and 5 days post treatment, followed by a sperm decrease between 15 and 30 days. Acridine orange method showed a significant increase of abnormal metachromatic spermatozoa in treated animals (red fluorescence). Testicular histology of treated animals showed tissue disorganization and vacuolization, with presence of small and hyperchromatic nuclei, indicative of possible apoptosis. It was also found that malathion caused body contraction characterized by coiled tail. Besides significant alteration in count and quality of spermatozoa were seen. Eisenia foetida is then an excellent model to monitor cytotoxic damage of organophosphate pesticides | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad Chilena de Anatomía | |
| dc.relation | 10.4067/S0717-95022004000400010 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | International Journal of Morphology v.22 n.4 2004 | |
| dc.subject | Eisenia foetida | |
| dc.subject | Malathion | |
| dc.subject | Cytotoxicity | |
| dc.subject | Spermatozoa | |
| dc.subject | Reproductive organs | |
| dc.title | SUBLETHAL DOSES OF MALATHION ALTER MALE REPRODUCTIVE PARAMETERS OF Eisenia foetida | |
