| dc.creator | JAIMOVICH,ENRIQUE | |
| dc.creator | ESPINOSA,ALEJANDRA | |
| dc.date | 2004-01-01 | |
| dc.date.accessioned | 2020-02-17T15:35:43Z | |
| dc.date.available | 2020-02-17T15:35:43Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/132049 | |
| dc.description | We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The different intracellular Ca2+ patterns produced by electrical stimulation, testosterone, IGF-I and insulin, may help to understand the role of intracellular calcium kinetics in the regulation of gene expression by various stimuli in skeletal muscle cells. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602004000400018 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.37 n.4 2004 | |
| dc.subject | skeletal | |
| dc.subject | transcription factors | |
| dc.subject | insulin | |
| dc.subject | IGF-I | |
| dc.subject | testosterone | |
| dc.title | Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells | |
