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dc.creatorWILHELM,VIVIAN
dc.creatorSOZA,CRISTIÁN
dc.creatorMARTÍNEZ,RODRIGO
dc.creatorROSEMBLATT,MARIO
dc.creatorBURZIO,LUIS O
dc.creatorVALENZUELA,PABLO D.T.
dc.date2005-01-01
dc.date.accessioned2020-02-17T15:36:23Z
dc.date.available2020-02-17T15:36:23Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/132407
dc.descriptionWe have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-97602005000100009
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceBiological Research v.38 n.1 2005
dc.subjectPiscirickettsia salmonis
dc.subjectHsp60
dc.subjectHsp70
dc.subjectgroEL
dc.subjectdnaK
dc.subjectimmune response
dc.titleProduction and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis


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