PHYTOCHEMICAL AND CRYSTALLOGRAPHIC STUDIES OF AZARA DENTATA EXTRACTS AND ITS CYTOTOXIC EFFECTS ON HUMAN BREAST CANCER CELL, MCF-7

Paz, Cristian; González-Chavarría, Iván; Freire, Eleonora; Ortiz, Leandro; Karpiński, Tomasz M.; Duprat, Felix; Baggio, Ricardo

dc.creator	Paz, Cristian
dc.creator	González-Chavarría, Iván
dc.creator	Freire, Eleonora
dc.creator	Ortiz, Leandro
dc.creator	Karpiński, Tomasz M.
dc.creator	Duprat, Felix
dc.creator	Baggio, Ricardo
dc.date	2020-09-10
dc.date.accessioned	2020-09-28T11:27:43Z
dc.date.available	2020-09-28T11:27:43Z
dc.identifier	http://www.jcchems.com/index.php/JCCHEMS/article/view/1532
dc.identifier.uri	https://revistaschilenas.uchile.cl/handle/2250/153703
dc.description	Azara dentata Ruiz & Pav. also called “Corcolen” is an endemic shrub of Chile. The honey produced in areas with abundance of Azara dentata is highly appreciate by its polyphenols. In the present work, we isolated and identified for the first time the phytochemical components of Azara dentata and its cytotoxic effects were analyzed on cancer cells together with its antimicrobial activity. The results showed that palmitic acid methyl ester, linolenic acid methyl ester, α-tocopherol and β-sitosterol are the main non-polar constituents of the plant, while the compounds AD-3 (3-phenylisocoumarin, 0.0001% yield) and AD-4 (methyl 2-phenacylbenzoate, 0.00039% yield) were isolated by column chromatography with n-hexane/EtOAc (3:2 v/v) and their structures were determined using NMR analysis. In addition, the structure of AD-4 was complemented by a single crystal x-ray structural determination. Azara dentata showed moderate antibacterial activity against S. aureus and S. epidermidis with a MIC of 5.0 and 10 mg/mL respectively. The cytotoxic activity of Azara dentata against MCF-7 cancer cells showed an IC50 of 15.63 µg/mL, this is a promissory value for the deeper study of its metabolites in cancer models.	en-US
dc.format	application/pdf
dc.language	eng
dc.publisher	Sociedad Chilena de Química	en-US
dc.relation	http://www.jcchems.com/index.php/JCCHEMS/article/view/1532/404
dc.rights	Copyright (c) 2020 SChQ	en-US
dc.rights	https://creativecommons.org/licenses/by-nc-sa/4.0	en-US
dc.source	Journal of the Chilean Chemical Society; Vol 65 No 3 (2020): Journal of the Chilean Chemical Society; 4891-4894	en-US
dc.source	0717-9707
dc.source	0717-9324
dc.subject	Azara dentata	en-US
dc.subject	Phytochemicals	en-US
dc.subject	Antimicrobial activity	en-US
dc.subject	Cytotoxic activity	en-US
dc.subject	X-Ray Diffraction	en-US
dc.subject	GC-MS.	en-US
dc.title	PHYTOCHEMICAL AND CRYSTALLOGRAPHIC STUDIES OF AZARA DENTATA EXTRACTS AND ITS CYTOTOXIC EFFECTS ON HUMAN BREAST CANCER CELL, MCF-7	en-US
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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