Show simple item record

dc.contributoren-US
dc.creatorCarrasco, Basilio
dc.creatorGarnier, Maurice
dc.creatorEaton, Lafayette
dc.creatorGuevara, Rafael
dc.creatorCarú, Margarita
dc.date2009-04-01
dc.date.accessioned2020-10-29T14:45:34Z
dc.date.available2020-10-29T14:45:34Z
dc.identifierhttps://www.rcia.uc.cl/index.php/ijanr/article/view/225
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/156689
dc.descriptionRandom Amplifi ed Polymorphic DNA (RAPD) markers were used to assess the genetic diversity of 587 individuals, belonging to 22 populations of Nothofagus nervosa that were distributed through the Coastal (38°S to 41°S) and Andes Mountains in Central- Southern Chile (36°S to 40°S). The objective of this study was to complement the genetic inferences previously determined by isozyme analysis, in order to obtain more accurate genetic diversity estimations. We scored 81.8% of the polymorphic loci of the samples tested. The average incidence of genetic polymorphism within populations was high, with values ranging between 33% and 63%. Analysis of molecular variance (AMOVA) showed most of the genetic variation was distributed within populations (87.6%), but FST values (FST = 0.124; p < 0.00001) indicated that there was also a signifi cant difference among populations. A discriminant analysis revealed three geographically defi ned groups and showed that 14 loci explained 87.2% of the genetic differentiation among N. nervosa populations. Watterson’s neutrality test and Ohta’s two-locus analysis of linkage disequilibrium (LD) both suggested that stochastic demographic and environmental factors can partially explain the loci variation observed in the RAPDs. The role of the last glaciations, as well as some conservation and breeding strategies, may have infl uenced current genetic variation and fragmentation in this species. Se evaluó la diversidad genética de 587 individuos pertenecientes a 22 poblaciones naturales de raulí (Nothofagus nervosa), distribuidas a lo largo de la Cordillera de la Costa (38°S to 41°S) y en la zona centro sur de Chile a través de la Cordillera de los Andes (36°S to 40°S). El objetivo de este estudio fue complementar las inferencias genéticas previamente determinada por isoenzimas, para obtener estimaciones mas adecuadas de la diversidad genética. A partir de las 33 bandas RAPD analizadas se observó un promedio de 88,1% de loci polimórfi cos con valores que fl uctuaron entre 33% y 63%. El análisis análisis de varianza molecular (AMOVA) reveló que la mayor proporción de la variación genética se encuentra distribuída dentro de las poblaciones estudiadas (87,6%). Sin embargo, el valor de FST (FST = 0.124; p < 0.00001) indicó que hay una signifi cativa diferenciación entre poblaciones. El análisis discriminante mostró la existencia de tres grupos geográfi cos defi nidos con 14 loci explicando el 87,2% de la diferenciación genética entre poblaciones. La prueba de neutralidad de Watterson y el análisis de desequilibrio de ligamiento (LD) de Ohta sugieren que la variación detectada podría ser explicada en parte por factores estocásticos demográfi cos y ambientales. El rol de las últimas glaciaciones así como algunas medidas de conservación y mejoramiento es discutido.en-US
dc.format
dc.languageeng
dc.publisherPontificia Universidad Católica de Chileen-US
dc.relationhttps://www.rcia.uc.cl/index.php/ijanr/article/view/225/169
dc.sourceInternational Journal of Agriculture and Natural Resources; Vol 36, No 2 (2009); 229-238en-US
dc.sourceInternational Journal of Agriculture and Natural Resources; Vol 36, No 2 (2009); 229-238es-ES
dc.source2452-5731
dc.source2452-5731
dc.subjecten-US
dc.subjectGenetic structure, Nothofagus, RAPD markers, Estructura genética, Nothofagus, marcadores RAPDen-US
dc.titleProximal causes of genetic variation between and within populations of raulí (Nothofagus nervosa).en-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


This item appears in the following Collection(s)

Show simple item record