New signal processing methods based on one-dimensional continuous wavelet transforms were proposed for the simultaneous quantification of chlorogenic acid and rutin in the leaves of Ribes uva-crispa L. These methods are based on the implementation of the continuous wavelet transform for the absorption spectral bands of the analyzed compounds and their samples. In the spectral wavelet analysis, symlets 8 and biorthogonal 4.4 continuous wavelet transforms (sym8-CWT and bior4.4-CWT) among several wavelet families were found to be optimal ones to get the best chemical determination of the related compounds in the analyzed plant. Linear regression lines were obtained by measuring sym8-CWT and bior4.4-CWT amplitudes at 333.5 and 336.0 nm for chlorogenic acid and 351.5 and 350.0 nm for rutin the spectral region 250-450 nm. Linear regression curves were found to be linear in the working range of 2.5-40.0 µg/mL for both compounds. The validation and analytical applicability of the proposed sym8-CWT approach were confirmed by analyzing the synthetics mixtures and standard addition samples. It was observed that the application of the sym8-CWT approach is very appropriate for the simultaneous spectral quantification of chlorogenic acid and rutin in the real plant samples consisting of leaves of Ribes uva-crispa L.