Mycoplasma isolation in milk samples from dairy herds in Chile

Ulloa, Fernando; Soto, Juan P.; Kruze, Juan; Mella, Armin

dc.creator	Ulloa, Fernando
dc.creator	Soto, Juan P.
dc.creator	Kruze, Juan
dc.creator	Mella, Armin
dc.date	2021-05-12
dc.identifier	http://revistas.uach.cl/index.php/australjvs/article/view/6530
dc.identifier	10.4067/S0719-81322021000200109
dc.description	Mycoplasma bovine mastitis is a highly contagious disease, usually associated with clinical cases refractory to antibiotic treatment. The aim of this study was the isolation of Mycoplasma species in cattle milk samples from dairy herds in Chile. Bulk tank milk samples selected by convenience from 91 Holstein Friesian dairy herds located in Los Rios (66) and Los Lagos (25), the two most important dairy Regions in the country, were collected. Additionally, 100 individual milk samples from cows with a high incidence of clinical mastitis, refractory to antibiotic therapy, and negative bacteriological results for traditional mastitis pathogens, all from the Biobío Region and received in our diagnostic laboratory, were included. All samples were cultured for 10 days on PPLO medium. The differentiation of suspect colonies between genus Mycoplasma and Acholeplasma was performed by the digitonin test and a specific PCR. The species identification was performed by a M. bovis specific PCR and 16S rRNA sequencing. Mycoplasma was isolated from 3 (3.3%) bulk tank milk samples and 2 (2%) individual cow milk samples. All colonies were identified as Mycoplasma by the digitonin test and by a specific PCR. At species level, one strain isolated from a bulk tank milk sample was identified as M. bovis. The remaining two strains isolated from bulk tank milk samples were identified as M. bovigenitalium, while the two strains isolated from milk of individual cows were identified as M. alkalescens. These results show that not only M. bovis is present in Chilean dairy herds, but also other pathogenic species not previously described in Chile such as M. bovigenitalium and M. alkalescens, which pose a potential risk for dairy herds in southern Chile.	en-US
dc.description	Mycoplasma bovine mastitis is a highly contagious disease, usually associated with clinical cases refractory to antibiotic treatment. The aim of this study was the isolation of Mycoplasma species in cattle milk samples from dairy herds in Chile. Bulk tank milk samples selected by convenience from 91 Holstein Friesian dairy herds located in Los Rios (66) and Los Lagos (25), the two most important dairy Regions in the country, were collected. Additionally, 100 individual milk samples from cows with a high incidence of clinical mastitis, refractory to antibiotic therapy, and negative bacteriological results for traditional mastitis pathogens, all from the Biobío Region and received in our diagnostic laboratory, were included. All samples were cultured for 10 days on PPLO medium. The differentiation of suspect colonies between genus Mycoplasma and Acholeplasma was performed by the digitonin test and a specific PCR. The species identification was performed by a M. bovis specific PCR and 16S rRNA sequencing. Mycoplasma was isolated from 3 (3.3%) bulk tank milk samples and 2 (2%) individual cow milk samples. All colonies were identified as Mycoplasma by the digitonin test and by a specific PCR. At species level, one strain isolated from a bulk tank milk sample was identified as M. bovis. The remaining two strains isolated from bulk tank milk samples were identified as M. bovigenitalium, while the two strains isolated from milk of individual cows were identified as M. alkalescens. These results show that not only M. bovis is present in Chilean dairy herds, but also other pathogenic species not previously described in Chile such as M. bovigenitalium and M. alkalescens, which pose a potential risk for dairy herds in southern Chile.	es-ES
dc.format	application/pdf
dc.language	eng
dc.publisher	Facultad de Ciencias Veterinarias - Universidad Austral de Chile	es-ES
dc.relation	http://revistas.uach.cl/index.php/australjvs/article/view/6530/7526
dc.rights	Derechos de autor 2021 Austral Journal of Veterinary Sciences	es-ES
dc.source	Austral Journal of Veterinary Sciences; Vol. 53 Núm. 2 (2021); 109-113	en-US
dc.source	Austral Journal of Veterinary Sciences; Vol. 53 Núm. 2 (2021); 109-113	es-ES
dc.source	0719-8132
dc.source	0719-8000
dc.title	Mycoplasma isolation in milk samples from dairy herds in Chile	en-US
dc.title	Mycoplasma isolation in milk samples from dairy herds in Chile	es-ES
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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