Aim: This work is aimed to the study the effect of aqueous extracts obtained from leaves of Ugni molinae and their respective products of in vitro gastrointestinal digestion on the viability of colon cancer cells. Methods: Ugni molinae leaves were used to prepare a 1%, aqueous extract and with this, an in vitro gastrointestinal digestion was performed, obtaining, fraction products of digestion gastrointestinal (Dgi) and fraction residue. this fraction was identified through liquid chromatography (HPLC-UV) and liquid chromatography-mass spectrometry (HPLC-MS), and then these samples were evaluated in different viability tests, such as trypan blue exclusion, metabolic activity 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and cytotoxicity by lactate dehydrogenase (LDH), on colorectal cancer cells (CaCo-2) and human embryonic kidney 293 cells (HEK) for a period of 24 and 48 hours. Results and discussion: The aqueous extracts of leaves of Ugni molinae and their digestion fractions caused inhibition of the viability of Caco-2 cells. In most cases, the effect was concentration-dependent and in particular, the only compound that generated inhibition of the viability in all trials corresponded to the Dgi fraction.  Conclusion: The results are favorable, since it is finally is this product that would act in the gastrointestinal tract, interacting with the target cells. These results are important since in most cases, these compounds had effects on cancer cells Caco-2 and not on HEK cells at the same concentrations used in each trial.