EVALUATION OF CYTOTOXIC EFFECT AGAINST TUMOUR CELLS OF THE ACIDIC POLYSACCHARIDES OF THE FUNGUS NOTHOPHELLINUS ANDINOPATAGONICUS

Cabrera Pardo, Jaime R; Albornoz , Verónica; Casas, Virginia; Figueroa, Fabián; Hernández, Victor; Perez, Claudia; Rajchenberg, Mario; Smith, Carlos; Becerra, José; Abdala-Díaz, Roberto; Campos, Víctor

dc.creator	Cabrera Pardo, Jaime R
dc.creator	Albornoz , Verónica
dc.creator	Casas, Virginia
dc.creator	Figueroa, Fabián
dc.creator	Hernández, Victor
dc.creator	Perez, Claudia
dc.creator	Rajchenberg, Mario
dc.creator	Smith, Carlos
dc.creator	Becerra, José
dc.creator	Abdala-Díaz, Roberto
dc.creator	Campos, Víctor
dc.date	2022-02-24
dc.identifier	http://www.jcchems.com/index.php/JCCHEMS/article/view/1997
dc.description	Fungal polysaccharides possess an important bioactive potential, including antioxidant and anticarcinogenic activity. The aim of this work was to determine the antioxidant activity and cytotoxicity against tumour and non-tumour cell lines acidic polysaccharides (NAAPs) of the fungus Nothophellinus andinopatagonicus. The effect of NAAPs on tumour cells lines was evaluated by MTT assay and flow cytometry. The analyses determined that glucose was the most abundant monomer and IR spectrum showed the typical peaks of β-glucans in the NAAPs. The cell viability assays revealed significant activity of NAAPs against HL-60, HCT-116 and MCF-7 tumour cell lines (IC50 = 767,16 µg mL-1, 1256 µg mL-1 and 4241,7 µg mL-1, respectively); but a much lower cytotoxicity against the non-tumour cell line HGF-1 (outside the range of the highest concentration tested (>10 mg mL-1)). NAAPs affected the cell cycle of HL-60 tumour cells, increasing the percentage of cells in the sub G1 phase and reducing it in the S/G2/M phases. Moreover, low concentrations of NAAPs also showed an effective cytotoxic activity against tumour cell lines while the non-tumour cell line was unaffected, maintaining a viability close to 100%. The antioxidant activity of the highest NAAPs concentration tested was 6.24% and 4.63%, for DPPH and ABTS method, respectively.	en-US
dc.format	application/pdf
dc.language	eng
dc.publisher	Sociedad Chilena de Química	en-US
dc.relation	http://www.jcchems.com/index.php/JCCHEMS/article/view/1997/526
dc.rights	Copyright (c) 2022 SChQ	en-US
dc.rights	https://creativecommons.org/licenses/by-nc-sa/4.0	en-US
dc.source	Journal of the Chilean Chemical Society; Vol 67 No 1 (2022): Journal of the Chilean Chemical Society; 5418-5424	en-US
dc.source	0717-9707
dc.source	0717-9324
dc.subject	acidic polysaccharides	en-US
dc.subject	fungi	en-US
dc.subject	Nothophellinus andinopatagonicus	en-US
dc.subject	anticarcinogenic activity	en-US
dc.subject	antioxidants	en-US
dc.subject	cell cycle	en-US
dc.title	EVALUATION OF CYTOTOXIC EFFECT AGAINST TUMOUR CELLS OF THE ACIDIC POLYSACCHARIDES OF THE FUNGUS NOTHOPHELLINUS ANDINOPATAGONICUS	en-US
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

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