Lower back pain (LBP) is recognized as a cause of morbidity in developed nations in different occupational situations. Health care workers (HCWs), in particular nurses, are especially vulnerable to LBP. About 60–80% of the general people suffer from LBP at some time during their lives. However, there is not enough care about workplace health and safety problems facing the health care workforce in developing nations, such as Libya. Thus, this study aimed to find out the status of low back pain and factors affecting pain among the nurses in Benghazi Medical Centre (BMC), Libya. Cross-sectional study was conducted on BMC in 2018. The data was collected by a self-administered questionnaire. Chi-square was used to determine the association between associated risk factors and LBP prevalence (p<0.05). There is a high prevalence (79%) of low back pain among nurses in BMC. Individual and work-related factors were found as risk factors for LBP. In Libya, nurses are considered as a critical health and safety concern, as a result of the weakness of policies in healthcare organizations. Consequently, a proper no weight lifting policy should be considered. If not, proper manual lifting must be implemented.