Detection of pathogenic leptospira as a cause of abortion in cattle-observations on diagnosis

Tomckowiack, Camilo; Matamala, Sebastián; León, Constanza; Moroni, Manuel; Salgado, Miguel

dc.creator	Tomckowiack, Camilo
dc.creator	Matamala, Sebastián
dc.creator	León, Constanza
dc.creator	Moroni, Manuel
dc.creator	Salgado, Miguel
dc.date	2022-05-09
dc.date.accessioned	2022-07-14T14:59:48Z
dc.date.available	2022-07-14T14:59:48Z
dc.identifier	http://revistas.uach.cl/index.php/australjvs/article/view/6761
dc.identifier	10.4067/S0719-81322022000200077
dc.identifier.uri	https://revistaschilenas.uchile.cl/handle/2250/197699
dc.description	Leptospirosis is a zoonotic infectious disease caused by members of the genus Leptospira, which affects domestic and wild animals. Cases of abortion in cattle have been associated with this infection, but these are often not adequately confirmed. To determine the best diagnostic strategy for leptospirosis-associated cases of abortion, we evaluated some of the techniques used in the veterinary laboratory and found that the key issues are sample type and timing. In a retrospective anatomical and histopathological analysis, we studied 42 aborted foetuses with lesions consistent with leptospirosis to check for the presence of pathogenic leptospira by qPCR, as well as ascertaining the serologic status of the cows. In addition, in a prospective analysis, cows that had aborted foetuses were analysed within 2 days of the event by MAT and qPCR using blood and urine samples. Analysis of the foetuses indicated that only 14.3% of the selected cases (6 of 42) gave a positive qPCR result. Regarding cows that had recently aborted foetuses, 4 out of 11 sampled showed a positive qPCR, while MAT tests showed only negative results. The evidence provided in this study indicates that the time that has elapsed since a clinical event has occurred and the type of clinical sample taken are key elements in the successful confirmation of pathogenic leptospira as the cause of abortion.	en-US
dc.description	Leptospirosis is a zoonotic infectious disease caused by members of the genus Leptospira, which affects domestic and wild animals. Cases of abortion in cattle have been associated with this infection, but these are often not adequately confirmed. To determine the best diagnostic strategy for leptospirosis-associated cases of abortion, we evaluated some of the techniques used in the veterinary laboratory and found that the key issues are sample type and timing. In a retrospective anatomical and histopathological analysis, we studied 42 aborted foetuses with lesions consistent with leptospirosis to check for the presence of pathogenic leptospira by qPCR, as well as ascertaining the serologic status of the cows. In addition, in a prospective analysis, cows that had aborted foetuses were analysed within 2 days of the event by MAT and qPCR using blood and urine samples. Analysis of the foetuses indicated that only 14.3% of the selected cases (6 of 42) gave a positive qPCR result. Regarding cows that had recently aborted foetuses, 4 out of 11 sampled showed a positive qPCR, while MAT tests showed only negative results. The evidence provided in this study indicates that the time that has elapsed since a clinical event has occurred and the type of clinical sample taken are key elements in the successful confirmation of pathogenic leptospira as the cause of abortion.	es-ES
dc.format	application/pdf
dc.language	eng
dc.publisher	Faculty of Veterinary Sciences - Universidad Austral de Chile	es-ES
dc.relation	http://revistas.uach.cl/index.php/australjvs/article/view/6761/7868
dc.rights	Derechos de autor 2022 Austral Journal of Veterinary Sciences	es-ES
dc.source	Austral Journal of Veterinary Sciences; Vol. 54 No. 2 (2022); 77-81	en-US
dc.source	Austral Journal of Veterinary Sciences; Vol. 54 Núm. 2 (2022); 77-81	es-ES
dc.source	0719-8132
dc.source	0719-8000
dc.subject	Leptospirosis	es-ES
dc.subject	pathogenic leptospira	es-ES
dc.subject	abortion	es-ES
dc.subject	dairy cattle	es-ES
dc.subject	diagnostic	es-ES
dc.subject	Leptospirosis	en-US
dc.subject	pathogenic leptospira	en-US
dc.subject	abortion	en-US
dc.subject	dairy cattle	en-US
dc.subject	diagnostic	en-US
dc.title	Detection of pathogenic leptospira as a cause of abortion in cattle-observations on diagnosis	en-US
dc.title	Detection of pathogenic leptospira as a cause of abortion in cattle-observations on diagnosis	es-ES
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion

This item appears in the following Collection(s)

Austral Journal of Veterinary Sciences
[0-9]{4}

Show simple item record