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dc.creatorUZAL,F.A.
dc.creatorSMITH,P.
dc.creatorNIELSEN,K.
dc.creatorLIN,M.
dc.creatorKELLY,W.R.
dc.date1999-01-01
dc.date.accessioned2022-08-29T19:34:02Z
dc.date.available2022-08-29T19:34:02Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X1999000100015
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/206005
dc.descriptionA fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation of ten measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later
dc.formattext/html
dc.languageen
dc.publisherFacultad de Ciencias Veterinarias, Universidad Austral de Chile
dc.relation10.4067/S0301-732X1999000100015
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceArchivos de medicina veterinaria v.31 n.1 1999
dc.subjectfluorescence polarization assay
dc.subjectgoats
dc.subjectClostridium perfringens
dc.subjectepsilon toxin antibodies
dc.titleA homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats


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