The bark of stone pine (Pinus pinea) from 50 year old trees grown in Portugal was submitted to grinding and fractioning into different particles sizes. The trees had a thick bark with an average 3,7 cm constituted mainly by the periderm and rhytidome (3,2 cm).The bark fractured easily into particles: yield of fines was low, and 74,0% of the particles were over 2 mm. The chemical composition, as a mass weighed average of all granulometric fractions showed a content of 1,1% ash 20,6% extractives (91% of which polar extractives) 2,2% suberin, 43,0% lignin and 37,6% holocellulose. The percentage of material dissolved by extraction with 1% NaOH was 42,3%. The chemical characterization of the different granulometric fractions showed that extractives were present preferentially in the finest fractions (<80 mesh and 60-80 mesh), representing 34-35%, particularly with enrichment in ethanol soluble extractives, that also showed lower content of lignin. The coarser fractions contained higher proportions of lignin and holocellulose. P. pinea bark grinding and fractionation by particle size may be used to selectively enrich the finest fractions in soluble materials, while the coarser fractions tend to have higher holocellulose content and will be therefore more suitable for carbohydrate related uses.