| dc.creator | Tapia, Rodrigo | |
| dc.creator | Medina, Rafael | |
| dc.creator | Neira, Victor | |
| dc.date | 2024-05-22 | |
| dc.date.accessioned | 2024-11-14T19:51:19Z | |
| dc.date.available | 2024-11-14T19:51:19Z | |
| dc.identifier | http://revistas.uach.cl/index.php/australjvs/article/view/7578 | |
| dc.identifier | 10.4206/ajvs.562.01 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/246031 | |
| dc.description | Novel swine Influenza A viruses (IAVs) have been described in South America. The objective of this study was to evaluate the replication kinetics of novel swine IAVs as a first step in vaccine production. Different swine IAV lineages (H1N1, H1N2, and H3N2), infection doses (MOI: 1, 0.1, 0.01, 0.001, 0.0001, and 0.00001), harvest times (every 12 h), and substrates (MDCK and Vero cells) were used. For all IAV strains, MDCK cells were the most efficient substrate, generating titers of ≥128 HAU/50 μL with an MOI of 0.00001 at 60 h post-infection. These data may be useful in vaccine-producing laboratories. | en-US |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Faculty of Veterinary Sciences - Universidad Austral de Chile | es-ES |
| dc.relation | http://revistas.uach.cl/index.php/australjvs/article/view/7578/8598 | |
| dc.source | Austral Journal of Veterinary Sciences; Vol. 56 No. 2 (2024); 85-90 | en-US |
| dc.source | Austral Journal of Veterinary Sciences; Vol. 56 Núm. 2 (2024); 85-90 | es-ES |
| dc.source | 0719-8132 | |
| dc.source | 0719-8000 | |
| dc.subject | Influenza A virus | en-US |
| dc.subject | MDCK cells | en-US |
| dc.subject | Vero cells | en-US |
| dc.subject | antigen | en-US |
| dc.subject | vaccine | en-US |
| dc.subject | swine | en-US |
| dc.title | Replication kinetics of novel swine influenza A viruses: an approach to vaccine production | en-US |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
