The objective of this work was to evaluate the in vitro and in silico antibacterial, anti-inflammatory and antioxidant activities of two oleoresins; Myrrh and Pine resin used in the Algerian traditional pharmacopoeia. The antibacterial effect of oleoresins was evaluated by the agar diffusion test against three bacterial strains; E. coli (ATCC 25922), S. aureus (ATCC 25923) and P. aeuroginosa (ATCC 27853). The antioxidant activity was assessed using DPPH method and the protein inhibition denaturation test was used to evaluate the anti-inflammatory efficacy. Resins main compounds were docked in silico against the bacterial tyrosyl-tRNA synthetase using the Autodock Tools 1.5.7 software. This study was carried out to determine their modes of binding with the active residues of this molecular target enzyme of antimicrobial agents. Molinspiration Cheminformatics and SwissADME online tools were used to predict physicochemical and pharmacokinetic parameters while OSIRIS Property Explorer online tools were used to predict toxicity risks. The results show that the Myrrh was effective against E. coli and S. aureus (17 mm) and that the Pine resin was similarly effective against E. coli (11 mm) and S. aureus (10 mm), but P. aeruginosa was completely resistant. The antioxidant test showed that both oleoresins had considerable ability to reduce the DPPH, with good IC50 of 0.49 ± 0.13 and 0.53 ± 0.06 mg/ml, respectively, compared to the BHT (0.89 ± 0.45 mg/ml). Both oleoresins had a remarkable anti-denaturation effects. The data of in silico studies revealed that all phytocompounds fit into the active pocket of the target enzyme and the binding energies ranged between -10.06 (Dehydroabietic acid) and -4.3 kcal/mol (D-glucuronic acid). The toxic and pharmacokinetic characteristics are, mostly, satisfying except for some compounds which have shown toxic effects, in particular Limonene, 4-allylanisole and Vanillin. We conclude that the extracts and their primary phytocompounds can enhance the antibacterial, antioxidant, and anti-inflammatory existing drugs without side effects.