Introduction: Infections and conditions involving the pulp tissue have been treated with Calcium hydroxide (CaOH) since long. In the last decade, use of Mineral trioxide aggregate (MTA) has gained ground. This study was carried out to comparatively evaluate the Ca release from CaOH powder with different vehicles and different types of MTA. Materials and Methods: 40 single rooted mandibular premolars were selected, decoronated and biomechanically prepared. They were randomly divided into four groups, consisting of 10 samples each. Root canals were packed with different preparations of CaOH and MTA. Ca ion released was evaluated with an UV-spectrophotometer. Result: Amongst the CaOH preparations, propylene glycol as a vehicle produced extended release of Ca ion (7.3410±.01135) for a period of 14 days. Whereas, amongst MTA based products, MTA angelus produced maximum Ca ions release (2.4240±.01075). Statistically significant difference was present between the four groups (p<0.05). Conclusion: Propylene glycol mixed with CaOH powder, produces higher and extended release of Ca ions than distilled water. MTA angelus produces better and consistent Ca ion release.