dc.contributor | | en-US |
dc.creator | Torres, Eillen | |
dc.creator | García, Apolinaria | |
dc.creator | Aranda, Mario | |
dc.creator | Saéz, Vania | |
dc.creator | Zúñiga, Felipe | |
dc.creator | Alarcón, Julio | |
dc.creator | Avello, Marcia | |
dc.creator | Pastene, Edgar | |
dc.date | 2019-01-09 | |
dc.date.accessioned | 2019-04-16T16:42:49Z | |
dc.date.available | 2019-04-16T16:42:49Z | |
dc.identifier | https://www.jcchems.com/index.php/JCCHEMS/article/view/920 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/42383 | |
dc.description | Avocado peels were used to extract proanthocyanidins (PACs) as starting material to prepare semi-synthetic derivatives of epicatechin by nucleophilic attack with pyrogallol and resorcinol. One-step isolation of both semi-synthetic derivatives was successfully performed through centrifugal partition chromatography (CPC), and identified by TLC and LC-MS/MS. The effect upon adherence of Helicobacter pylori ATCC 43504 to AGS cells and the subsequent induction of pro-inflammatory IL-8 release was tested for these two semi-synthetic derivatives. Bacterial adherence and IL-8 synthesis was evaluated using AGS cells con-infected with FITC-labeled H. pylori. Concentration of 700 μg/mL of epicatechin-pyrogallol and epicatechin-resorcinol decreased H. pylori adherence to AGS cells to less than 20% and reduced IL-8 production to less than 10%. In conclusion, semi-synthetic adducts prepared from avocado PACs reduce significantly the adherence and IL-8 production in H. pylori-infected AGS cells. | en-US |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | Sociedad Chilena de Química | en-US |
dc.relation | https://www.jcchems.com/index.php/JCCHEMS/article/view/920/281 | |
dc.rights | Copyright (c) 2019 Journal of the Chilean Chemical Society | en-US |
dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0 | en-US |
dc.source | Journal of the Chilean Chemical Society; Vol 63, No 4 (2018): Journal of the Chilean Chemical Society | en-US |
dc.source | 0717-9707 | |
dc.subject | Centrifugal Partition Chromatography; Helicobacter pylori; avocado; proanthocyanidins; IL-8 | en-US |
dc.title | ONE-STEP PURIFICATION OF TWO SEMI-SYNTHETIC EPICATECHIN ADDUCTS PREPARED FROM AVOCADO PEELS PROCYANIDINS BY CENTRIFUGAL PARTITION CHROMATOGRAPHY AND EVALUATION OF THEIR ANTI-INFLAMMATORY EFFECTS ON ADENOCARCINOMA GASTRIC CELLS INFECTED WITH Helicobacter pylori | en-US |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | | en-US |