Salmo trutta fario L. 1758, presented a chromosome number 2n = 80 and a fundamental number NF = 100. Thehaploid chromosome set consists of seven metacentric chromosomes, three submetacentric, one subtelocentric and 29 telocentrics. The Ag-NOR are located at conserved position in the short arm of pair 11 (st), with the presence of additional NOR on the short arm of pair 9 (sm) which is rare in others taxa previously studied.