We present the first observations of an arboreal bothriurid scorpion and the first report of an arboreal scorpion from the temperate forests of southern Chile. Urophonius tumbensis Cekalovic 1981 was observed and captured with ultraviolet (UV) light at a height of up to 6 meters on trunks of Cryptocarya alba (Molina) Looser trees in the Hualpén Botanical Reserve (Bío Bío Region, Chile).