The study objective was to determinate the concentration of aluminum that allow to distinguish genotypes of Jatropha for tolerance to this element, using the solution-paper method. For this study, seeds of four genotypes of Jatropha, were germinated at different Al concentrations (0, 15, 40, 80 and 120 mg L-1). After 14 days, the root length was measured for all treatments. It was observed that there are different tolerance levels for the genotypes. The root length decreased exponentially with the increase of the Al concentration. The solution-paper method allows the selection of genotypes of Jatropha in relation to aluminum tolerance and the concentration of 40 mg L-1 of Al is recommended to discriminate the tolerance of genotypes to this element.