To compare the wheat genotypes in terms of seed germination and growth of seedling under osmotic stress conditions, a laboratory experiment was conducted using a completely random design with factorial arrangement. The wheat genotype (G) consisted of eight genotypes numbered as (G1) 9103, (G2) 9116, (G3) 9203, (G4) 9205, (G5) 9207, (G6) 9212, (G7) C-81-10 and (G8) cross-shahi. The osmotic potential factor (Ψs) consisted of five levels (-1.2,-0.9,-0.6,-0.3 and 0 MPa). Genotypes G2, G6 and G7 prevailed in terms of germination percentage (GP) up to Ψs = -1.2, while genotype G8 and genotype G6 were only prevalent at Ψs = 0 and up to Ψs = -0.9, respectively. The two genotypes of G1 and G4 had lower GPs at Ψs = 0 compared to other genotypes but these two genotypes had no significant difference with resistant genotype G7 in terms of GP at Ψs = -0.3. This trend continued in case of genotype G1 up to Ψs = -1.2, while genotype G4 had the lowest GP value next to G8 cultivar from Ψs = -0.6 to Ψs = -1.2. Interaction effect of Ψs x genotype was significant for Coleoptile Length (CL), Radicle Length (RL), Plumule Length (SL). G7 prevailed in terms of CL with a significant difference at Ψs = -1.2 while G1 lost its dominance. The same trend was observed in interaction effect of Ψs x genotype for SL. According to GP and CL and SL values, modern genotype G7 was the most resistant genotype to osmotic stress while old genotype G8 was the most sensitive one.