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dc.creatorFernandes Boaro Martins,Márcia Regina
dc.creatorCarvalho Pinto e Silva,José Ricardo de
dc.date2005-01-01
dc.date.accessioned2019-04-25T12:33:56Z
dc.date.available2019-04-25T12:33:56Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400007
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/59398
dc.descriptionThe objective of this study was to describe some changes in spermatids differentiating events during spermiogenesis, leading to spermatozoa final formation. The animals were sacrificed with ethylic acid inhalation and, then, testis fragments were collected, fixed in Karnovsky and post-fixed in osmium tetroxide. After dehydration, the sections were stained with uranyl acetate 2%. It was observed in early spermatids at Golgi phase, formation of acrosomal granule contained in the acrosomal vesicle, spheroidal nucleus with rugous reticulum around it, and Golgi apparatus made up of lamellae and vesicular structures. At cephalic hood phase, the granule suffers flattening and the marginal fossa is formed. In both phases, it was possible to observe the presence of multivesicular body. At acrosomal phase, the acrosomal system is set up, and several enzymes of endoplasmatic reticulum as well chromatoid body are observed in the cytoplasm. At the last phase, that is, maturation, the nucleus becomes more elongated, the endoplasmatic reticulum regresses, the Golgi apparatus approaches to the flagellum and mitochondrias align next to it
dc.formattext/html
dc.languageen
dc.publisherSociedad Chilena de Anatomía
dc.relation10.4067/S0717-95022005000400007
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceInternational Journal of Morphology v.23 n.4 2005
dc.subjectSpermiogenesis
dc.subjectSpermatids
dc.subjectMice
dc.titleUltrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J


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