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dc.creatorVenegas,Felipe
dc.creatorGuillomot,Michel
dc.creatorVignon,Xavier
dc.creatorServely,Jean-Luc
dc.creatorAudouard,Chistophe
dc.creatorMontiel,Enrique
dc.creatorLe Bourhis,Daniel
dc.creatorPerón,Sandrine
dc.creatorSoto,Pilar
dc.creatorRojas,Mariana
dc.date2006-06-01
dc.date.accessioned2019-04-25T12:34:13Z
dc.date.available2019-04-25T12:34:13Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/59461
dc.descriptionThe objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid zone. The fusion of cell membranes is achieved by means of electric shocks. In respect to the timetable, we have observed that a stage of two blastomers is reached on the second day, and morulae of 8 to 16 cells on the third day. On the fourth day it has differenciated as a blastocyst and on the seventh day it finally hacht from the pellucid zone. The obtainment of embryonic blastocytes indicates that it is possible to obtain pudu embryos through heterospecific cloning, even though the percentage of success is relatively low. The viability of the embryos obtained in this manner after in uterus transfer remains still to be verified
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dc.languageen
dc.publisherSociedad Chilena de Anatomía
dc.relation10.4067/S0717-95022006000300026
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceInternational Journal of Morphology v.24 n.2 2006
dc.subjectPudu deer
dc.subjectCervidae
dc.subjectRuminant
dc.subjectCloning
dc.titleObtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique


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