Morphoquantitative Analysis of the Intraepithelial Neoplasic Uterine Cells by Computer Image System
Author
Lima,Jacqueline Silva Brito
Bittencourt,Alexandre Motta
Lima,Carlos Eduardo de Queiroz
Mota,Diógenes Luis da
Romaguera,Ana Maria de Ataíde
da Silva,Luiz Lúcio Soares
Abstract
The purpose of the present research was to detect the DNA variations by Feulgen stained chromatin and the packing degree of the cervical chromatin in the clinical cases of human uterine carcinomas. Scanning microphotometry was carried out on slides prepared from block Feulgen-stained. Accurate control procedures were performed using normal human cell nuclei. The cervical cells was morphometric studied using the Image Lab System for citologic classification. The overall diagnostic accuracy for neoplasia type I (CIN I) and type II (CIN II), demonstrated by stain interpetration values of a sensitive detection of DNA neuroploidy and a significant difference between the two groups in scanning static cytophotometry.