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dc.creatorPalma,R
dc.creatorMutis,A
dc.creatorManosalva,L
dc.creatorCeballos,R
dc.creatorQuiroz,A
dc.date2012-01-01
dc.date.accessioned2019-04-25T12:39:54Z
dc.date.available2019-04-25T12:39:54Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-95162012000100015
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/60990
dc.descriptionRoot volatiles from field-collected red clover plants, Trifolium pratense L., of five different ages were trapped by solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). Ethanol, E-2-hexenal, hexanal, 3-octanone, limonene and a-pinene were identified. The electroantennographic (EAG) and olfactometric responses of clover root borer, Hylastinus obscurus, to the identified compounds were studied. The GC-MS with SPME as a solvent-free collection method identified ethanol and hexanal compounds that have not previously been reported in T. pratense. The EAG experiments showed that all of the tested compounds were perceived by H. obscurus, but mixed responses were found in behavioral assays. For females, ethanol and E-2-hexenal were attractive at one or more of the tested doses, while hexanal, 3-octanone, R-limonene and S-limonene were repellent at one or more of the tested doses. Females responded to a larger number of tested compounds and a wider range doses compared to males. This behavior could reflect a more active role of females in host finding and colonization.
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dc.languageen
dc.publisherChilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo
dc.relation10.4067/S0718-95162012000100015
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceJournal of soil science and plant nutrition v.12 n.1 2012
dc.subjectRoot volatiles
dc.subjectred clover
dc.subjectroot borer
dc.subjectelectroantennography
dc.subjectolfactometry
dc.subjectSPME
dc.titleBehavioral and electrophysiological responses of Hylastinus obscurus to volatiles released from the roots of Trifolium pratense L.


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