A pot experiment was conducted to investigate the potential effect of arbuscular mycorrhizal fungi (Glomus mosseae & Acaulospora laevis) and phosphate solubilizing bacteria (Pseudomonasfluorescens) with different levels of superphosphate on Chrysanthemum indicum L. After 100 days, different plant growth parameters such as mycorrhization's characteristics, phosphatase activity and phosphorus uptake were measured. The obtained results revealed that the inoculation of plants with biofertilizers and recommended dose of superphosphate significantly improved the growth parameters. Inoculation with A. laevis + P. fluorescens at medium concentration of superphosphate showed maximum height, fresh and dry root weight, AM root colonization, AM spore count, alkaline phosphatase activity, acidic phosphatase activity and the percent phosphorus uptake in shoot and root whereas root length was maximum in G. mosseae + A. laevis + P.fluorescens. Leaf area and fresh and dry shoot weight were maximum in the treatment (G. mosseae + A. laevis + P. fluorescens) at low concentration of superphosphate. The use of AMF increased nutrient acquisition from an organic fertilizer source by enhancing acidic phosphatase (ACP) and alkaline phosphatase (ALP) activity, thus facilitating P acquisition and improving plant growth.