| dc.creator | Sasirekha,B | |
| dc.creator | Shivakumar,S | |
| dc.date | 2012-12-01 | |
| dc.date.accessioned | 2019-04-25T12:40:05Z | |
| dc.date.available | 2019-04-25T12:40:05Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-95162012000400018 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/61043 | |
| dc.description | The present study was undertaken to study the statistical optimization of medium components for improved Indole-3- acetic acid (IAA) production by Pseudomonas aeruginosa. Colorimetric analysis showed maximum IAA (132 ìg mL-1) in the medium supplemented with tryptophan (0.5 g L-1). The maximum production was achieved after 96 h of incubation. Yeast extract, tryptophan and EDTA were identified as significant components influencing IAA production by Pseudomonas aeruginosa, using the Plackett- Burman method. The statistical optimization approach led to the production of 318 ìg mL-1 of IAA within 24 h of incubation. Statistical approach was found to be very effective in optimizing the medium components in a manageable number of experimental runs with overall 2.4 fold increase in IAA production. TLC and GC- MS analysis further confirmed the IAA production in the cell filtrates of the strain. GC-MS analysis and tryptophan side chain oxidase confirmed the existence of atleast 2 possible pathways for IAA by this strain. Inoculation of P. aeruginosa culture filtrate enhanced seed germination (82.4%) and increase in root length and shoot length (~ 2.6 and ~ 1.1 folds over the control) of cowpea seeds over the control treatment under pot culture conditions. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Chilean Society of Soil Science / Sociedad Chilena de la Ciencia del Suelo | |
| dc.relation | 10.4067/S0718-95162012005000038 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Journal of soil science and plant nutrition v.12 n.4 2012 | |
| dc.subject | indole-3- acetic acid | |
| dc.subject | Plackett- Burman Design | |
| dc.subject | Pseudomonas aeruginosa | |
| dc.subject | GC- MS | |
| dc.subject | seed bacterization | |
| dc.title | Statistical optimization for improved indole-3-acetic acid (iaa) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion | |
