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dc.creatorLÓPEZ-SOLÍS,REMIGIO
dc.creatorPUENTE,MARGARITA
dc.creatorDURÁN,VIVIANA
dc.creatorMORALES-BOZO,IRENE
dc.creatorKEMMERLING,ULRIKE
dc.creatorPARDO,RODRIGO
dc.creatorWENK,CAROLINA
dc.date2001-03-01
dc.date.accessioned2019-04-25T12:52:56Z
dc.date.available2019-04-25T12:52:56Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000023
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/62467
dc.descriptionMouse salivary secretion provoked by single or multiple oral administrations of the cholinergic agent pilocarpine was characterized. An accessory ad hoc device, manipulated by a single operator, was used to collect saliva from various mice simultaneously. A single challenge by pilocarpine in the range 40-400 µg provoked a dose-dependent secretory response. The secretory response lasted for about 40 minutes and was constituted by four clearly defined stages, namely: lag (5-10 min), maximal flow (10 min), slowering (15-20 min) and postsecretory rest. In this response, usual parameters were: maximal flow rate, 30-40 µl/min; total volume of saliva, 250-350 µl/mouse and total salivary protein, about 700 µg/mouse. Temporal desensitization of the secretory response was observed within the first hour following a single stimulation by pilocarpine. However, recurrent stimulations by this agonist given at 24-h intervals produced an equally intense secretory response, thus suggesting resensitization during that period. The polypeptide composition of salivas obtained from a number of mice after a first pilocarpine stimulation were undistinguishable from each other. That salivary polypeptide pattern was also observed in a series of salivas obtained day by day from single animals stimulated at 24-h intervals by the agonist. Thus, both the characteristics of the secretory response as well as the polypeptide composition of mouse saliva after short-term or long-term challenges by pilocarpine were found to be highly consistent. Accordingly, these studies open the possibility of accomplishing a systematic molecular typing of saliva from individual living mice either from natural populations or from mice subjected to experimental laboratory conditions
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.relation10.4067/S0716-078X2001000000023
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista chilena de historia natural v.74 n.1 2001
dc.subjectmouse
dc.subjectsaliva
dc.subjectsecretion
dc.subjectpilocarpine
dc.subjectpolypeptides
dc.titleCharacterization of mouse salivary polypeptide secretion after oral administration of pilocarpine


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